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The temperature dependence profiles of the wild-type and mutant RsPtxD proteins. Each protein’s activity was measured using NAD (filled circles) or NADP (open circles) as a nicotinamide cofactor. The data are shown as means ± standard deviation obtained from three independent experiments. WT, wild-type; AR, RsPtxDAR; HAR, RsPtxDHAR; HARKA, RsPtxDHARKA; HARRA, RsPtxDHARRA.
