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. 2021 Apr 1;9:647176. doi: 10.3389/fbioe.2021.647176

FIGURE 5.

FIGURE 5

Shikimic acid (SA) production by shikimate dehydrogenase with NADPH regeneration by RsPtxD. (A) A schematic of the coupled reactions of SA production by shikimate dehydrogenase (SDH) and NADPH regeneration by RsPtxD. (B) The batch production of SA from 3-dehydroshikimate by SDH with NADPH regeneration by wild-type RsPtxD (diamonds), RsPtxDAR (squares), and RsPtxDHARRA (triangles). The reaction solutions contained 20 mM phosphite, 8 mM 3-DHS, 0.1 mM NADP, 0.17 U/mL of SDH, and 20 μg/mL of RsPtxD proteins.