FIGURE 2.

Effect of p21 knockdown in A549 cells on the levels of the TCA cycle and affiliated enzymes. (A–F) Activities of the enzymes indicated on Y axes are shown as μmol⋅ min^–1⋅ mg^–1; (G) protein level of the mitochondrial aspartate aminotransferase (GOT2), arbitrary units; (H) ratio of activities of GDH to total OGDHC, assessing the maximal glutamate degradation in the TCA cycle. The data are obtained from three independent cell cultures. Values are presented as mean ± SEM. Statistical significance of the differences is determined according to the Mann-Whitney test. Different p-values are shown by asterisks and mesh-sign as described in Materials and Methods under Statistical Analysis section.