FIGURE 4.

Changes in the levels of the enzymes and p53 in A549^WT and A549^p21– cells, induced by thiamine deficiency. (A,C,E–H) Activities of the enzymes indicated on Y axes are shown as μmol⋅ min^–1⋅ mg^–1; (B) protein level of the mitochondrial aspartate aminotransferase (GOT2), arbitrary units; (D) ratio of activities of GDH and endogenous holo-OGDHC (h-OGDHC), assessing the glutamate degradation in the TCA cycle; (I) protein level of p53, arbitrary units. Thiamine deficient (TD) cells were grown for 7 days in thiamine-free medium. The data are obtained from three independent cell cultures. Values are presented as mean ± SEM. Statistical significance of the differences is determined by two-way ANOVA followed by Tuckey’s post hoc test. P-values of the significances of the two factors and their interaction, analyzed by ANOVA, are provided in the table under the graphs. On the graphs, different p-values are shown by asterisks as described in Materials and Methods under Statistical Analysis section.