FIGURE 4.

SERCA2a-dependent [Ca²⁺]c overload triggered Mfn2-mediated [Ca²⁺]m overload and was accompanied by increased Drp-1-dependent mitochondrial fission (n = 3/group). (A,B) A Mito-ROS probe (MitoSOX) was used to compare the mito-oxidative stress levels between the different groups. (C–E) ELISA and tests on extracted mitochondria were performed to detect the function of mitochondria in the different groups. (F,G) Coimmunofluorescence of mitochondria and lysosomes in CMECs. The overlap of fluorescence intensity changed in the different groups to reveal the morphology of mitochondria and the function of lysosomes. (H,I) Western blot analysis was performed to detect Drp-1- and Fis-1-dependent mito-fission expression in different groups. *p < 0.05 compared with the Ad-Ctrl; ^#p < 0.05 compared with the Hypo(Ad-Ctrl); ^&p < 0.05 compared with the Hypo(Ad-SERCA).