Skip to main content
. 2021 Apr 3;7(4):e06617. doi: 10.1016/j.heliyon.2021.e06617

Figure 4.

Figure 4

Taqman® hPSC Scorecard™ Panel analysis based on embryoid bodies (EBs) spontaneously differentiated from clonal LCL-iPSC lines. Four clonal LCLs were spontaneously differentiated for 7 and 14 days into EBs. Total RNA was isolated from each EB using the RNeasy Blood and Tissue Kit (Qiagen). cDNAs were made using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) and applied to the 96-well Scorecard Panel. The panel contains probes to detect and report on markers of pluripotency and on trilineage potential (to ectoderm, mesoderm, and endoderm). (A) representative images (10x) of embryoid bodies at Day 2 (left image) and Day 3 (right image). Results of the day 7 and 14 time points are depicted in two formats: (B) Table of Scorecard™ values. All lines had downregulation of pluripotency markers (blue). Ectoderm and mesoderm gene expression were upregulated (red) in all cell lines at day 7 and 14. Endoderm gene expression was upregulated in all cell lines by day 14. (C) Box plot of Taqman® hPSC scores relative to reference data from undifferentiated cells.