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. 2021 Apr 3;7(4):e06617. doi: 10.1016/j.heliyon.2021.e06617

Table 1.

Primers used for PCR to assess pluripotency in clonal iPSC lines.

Primer Set Forward (5′-3′) Reverse (5′-3′) Size (bp)
EBNA-1 ATCGTCAAAGCTGCACACAG CCCAGGAGGTCCCAGTAGTCA 665
Β-actin CCCAGGCACCAGGGCGTGAT TCAAACATGATCTGGGTCAT 178
Oct3/4 CGTGAAGCTGGAGAAGGAGAAGCT CAAGGGCCGCAGCTCACACATGTTC 250
Nanog CAGCCCCGATTCTTCCACCAGTCCC CAGCCCCGATTCTTCCACCAGTCCC 400
DMNT3B TGCTGCTCACAGGGCCCGATACTTC TCCTTTCGAGCTCAGTGCACCACAAAAC 242
PODXL TCCAGCCCCACAGCAGXATCAACTACC CCGGGTTGAAGGTGGCTTTGACTGCTC 226