Table 2.
Basic Criteria necessary for patient-derived iPSCs to be considered adequate for further differentiation protocols. Three out of four LCL-derived iPSC clonal lines (Line 1-iPSC1A, Line 2-iPSCA2A, Line 2-iPSC2B) generated using the protocol described in this study meet all of these criteria and could be used for ectoderm-lineage differentiation. “X” denotes the iPSC line met the criterion in each column.
| LCL-Derived iPSC Lines | iPSC Morphology | Early Tra-1-60 Positive Staining | Efficiency of Passaging | Endogenous Gene Expression of Pluripotency Markers | Normal Karyotype | Spontaneous Differentiation |
|---|---|---|---|---|---|---|
| Line 1 iPSC 1A | X | X | X | X | X | X |
| Line 1 iPSC 1B | X | X | X | |||
| Line 2 iPSC 2A | X | X | X | X | X | X |
| Line 2 iPSC 2B | X | X | X | X | X | X |