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. 2021 Mar 1;296:100491. doi: 10.1016/j.jbc.2021.100491

Figure 7.

Figure 7

ssSPTb knockout shows normal hematopoiesis. A, real-time qPCR analysis of ssSPTb mRNA expression in BM cells isolated from ssSPTb+/+ and ssSPTb−/− mice. Gene expression was normalized to β-actin. qPCR results from three independent experiments. B, fluorescence activated cell sorting plots of myeloid bone marrow cells analyzed by Mac-1 and Gr-1 staining (n = 5). C, the total numbers of Mac-1+Gr-1+ were plotted for the ssSPTb+/+ and ssSPTb−/− mice (n = 5). D, the total numbers of CD71+Ter119+ cells, were plotted for the ssSPTb+/+ and ssSPTb−/− mice (n = 5). E, fluorescence activated cell sorting plots of LSK and LK populations of HSPCs from bone marrow of ssSPTb+/+ and ssSPTb−/− were analyzed by Sca-1 and c-Kit staining. F–I, the total numbers of LSK, LT-HSCs, ST-HSCs, and MPPs were plotted for the ssSPTb+/+ and ssSPTb−/− (n = 5). J–M, The total numbers of LK, CMPs, GMPs, and MEPs cells were plotted for the ssSPTb+/+ and ssSPTb−/− (n = 5). All graphs are represented as mean ± SEM. ∗p Value < 0.05 is significant, calculated from unpaired t test. CMP, common myeloid precursor; GMP, granulocyte-macrophage precursor; LK, Lin− c-Kit+ Sca1−; LSK, Lin−c-Kit+, Sca1+; LT-HSCs, long-term hematopoietic stem cells; MEP, megakaryocyte-erythrocyte precursor; MPPs, multipotent progenitor cells; ST-HSCs, short-term hematopoietic stem cells.