Primer sequences for real time RT-PCR
ssSPTa	Reverse: 5′-AGGTGGGCTGGCGTTAT-3′
	Reverse: 5′-CTGGGGCATGAAGACGTAGC-3′
ssSPTb	Forward: 5′-CGTGAAGGAGTATTTTGCCTGG -3′
	Reverse: 5′-GCCACAATGGTCAGTATGATGGT-3′
Nestin	Forward: 5′-TGAGGGTCAGGTGGTTCTG-3′
	Reverse: 5′-AGAGCAGGGAGGGACATTC-3′
Sox 17	Forward: 5′-AAGAAACCCTAAACACAAACAGCG-3′
	Reverse: 5′-TTTGTGGGAAGTGGGATCAAGAC-3′
Gsc	Forward: 5′-AAACGCCGAGAAGTGGAACAAG-3′
	Reverse: 5′-AAGGCAGGGTGTGTGCAAGTAG-3′
GAPDH	Forward: 5′-ACCATCTTCCAGGAGCGAG-3′
	Reverse: 5′-TAAGCAGTTGGTGGTGCAG-3′
Actin	Forward: 5′-TTCTTTGCAGCTCCTTCGTT-3′
	Reverse: 5′-ATGGAGGGGAATACAGCCC-3′
Brachyury	Forward: 5′-CGGACAATTCATCTGCTTG-3′
	Reverse: 5′-AGGTGGGCTGGCGTTAT-3′