TABLE 1.
The primer sequences of target genes.
| Primer | Sequence (5′–3′) |
| PCNA-F | GCTCCATCCTGAAGAAGGTGCTG |
| PCNA-R | CGTGGGACGAGTCCATGCTTTG |
| COL2A1-F | GTCCTGTGCGACGACATAATCT |
| COL2A1-R | GGCAGTGGCGAGGTCAGTAG |
| ACAN-F | GCTACGACGCCATCTGCTACAC |
| ACAN-R | GTCCTCCTCACCGCCCACTC |
| SOX9-F | GAAGCTCTGGAGACTGCTGAA |
| SOX9-R | CCCATTCTTCACCGACTTCCT |
| MMP13-F | TCCAGTTTGCAGAGAGCTACC |
| MMP13-R | GACTGCATTTCTCGGAGCCT |
| RUNX2-F | GAACCCAGAAGGCACAGACAGAAG |
| RUNX2-R | GAGGCGGGACACCTACTCTCATAC |
| ATX-F | TGTCCTCCTTCATCCTGCCTCAC |
| ATX-R | TGTTCAATGTCACGCACCCTAGC |
| RHOA-F | ATTGTCGGTGATGGTGCTTGTGG |
| RHOA-R | TGGGGACATACACCTCTGGGAAC |
| LATS1-F | GTGACCATCCACGGCAAGATAGC |
| LATS1-R | GTGCTAGACATCGCTGGTGCTG |
| LATS2-F | CCAACTCCTTCAACAGCCAGCAG |
| LATS2-R | CAGCACCCGCACACTCTTCAC |
| AKT2-F | GGTCGCCAACAGCCTCAAGC |
| AKT2-R | ACCGCCACTTCCATCTCCTCAG |
| AKT3-F | ACAGATGCAGCCACCATGAAGAC |
| AKT3-R | GAACGGCAACCTCCCACACATC |
| GAPDH-F | AGGTCGGAGTGAACGGATTT |
| GAPDH-R | GATCTCGCTCCTGGAAGATGG |
PCNA, proliferating cell nuclear antigen; COL2A1, type II collagen; ACAN, aggrecan; SOX9, SRY-related high-mobility group box 9; MMP13, matrix metalloproteinase 13; RUNX2, RUNX family transcription factor 2; ATX, autotaxin; RhoA, ras homolog family member; ALATS 1/2, large tumor suppressor kinase 1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.