Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 1;9:654654. doi: 10.3389/fcell.2021.654654

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Albashari, He, Albaadani, Xiang, Ali, Hu, Zhang, Zhang, Luo, Wang and Ye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

In vitro loading and release tests of modified Ti samples. (A) Heparin loading assay: samples were immersed in 1 ml of phosphate-buffered saline (PBS) (pH 7.4) containing 0.005% toluidine blue solution for 40 min, and solutions were measured at 620 nm. TNT/PDA/Ag surface showed the highest heparin loading capacity. (B) Bovine serum albumin (BSA) release of modified Ti samples: a total of 500 μg of BSA per modified Ti samples was added and left for 2 h. The adsorbed amount of BSA was quantified using a bicinchoninic acid kit (BCA) kit assay (570 nm). TNT/PDA/Ag/HP showed the highest protein adsorption among all modified surfaces. (C) Cumulative release of basic fibroblast growth factor (bFGF) from modified Ti samples in PBS (pH: 7.4) at 37°C measured by ELISA. Both TNT/PDA/Ag and TNT/PDA/Ag/HP displayed a slow release, with the latter being even more slowly. TNT, TNT/PDA/Ag, and TNT/PDA/Ag/HP reached the release peak on days 9, 12, and 15, respectively. Data are presented as mean ± standard deviation; ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001.