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. 2021 Apr 1;12:620427. doi: 10.3389/fimmu.2021.620427

Figure 1.

Figure 1

Co-ligation of complement receptor type 2 (CR2) with suboptimally stimulated B-cell receptor (BCR) elevates the Ca2+ response of human B cells. Resting human B cells were treated with complexes consisting of either streptavidin-conjugated anti-immunoglobulin G (IgG)/A/M (control samples) or streptavidin-linked anti-IgG/A/M and C3d molecules in different concentrations as indicated. Changes in fluorescence intensity were assessed for 300 s via flow cytometry and the Ca2+ response kinetics were examined. Response curves demonstrate the result of one representative experiment depicting mean fluorescence intensity as a function of time (A). The peak amplitude of each Ca2+ response curve was presented as a relative value compared to the peak amplitude of the control samples. Diagrams show cumulative results obtained in three independent experiments displaying the mean of the relative values ± SD (B). ***p < 0.001, ****p < 0.0001.