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. 2021 Apr 1;12:605212. doi: 10.3389/fimmu.2021.605212

Figure 5.

Figure 5

PMMA-CVVH treatment modulated TLR-4 signaling and reduced sCD40L and sCD40 sera levels. (A) ELISA assay showed significantly increased level of serum LBP in endotoxemic pigs after 3 and 24 h from LPS infusion respect to healthy group. A strong reduction in serum LBP levels was found after PMMA-CVVH treatment (#p < 0.05 vs LPS, and £p < 0.05 vs PS). PS-CVVH did not modulate LBP sera levels. (B, C) ELISA assay showed significantly increased level of serum sCD40L (B) and sCD40 (C) in endotoxemic pigs after 3 and 24 h from LPS infusion respect to healthy group. A significant strong decrease in serum sCD40L and sCD40 levels was found after PMMA-CVVH treatment (#p < 0.05 vs LPS, and £p < 0.05 vs PS). PS-CVVH reduced sCD40L and sCD40 sera levels but it did not reach the basal condition as with PMMA treatment. (A–C) The histograms represent the median ± IQR of at least five animals for each group (Healthy group and PMMA group, n = 7 for all time points; LPS group, T0-T3 n = 7, T10 -T24 n = 5; PS group, T0-T3-T10 n = 7 and T24 n = 6). Statistically significant differences were assessed by the Mann–Whitney test (*p < 0.05 vs Healthy, #p < 0.05 vs LPS, and £p < 0.05 vs PS).