Table 2.
Swarming, swimming, and twitching motility in reference strains (PAO1 and PA14), environmental isolates, and clinical isolates of Pseudomonas aeruginosa in the presence and absence of biogenic silver nanoparticles (bio-AgNPs) at ½ MIC.
| Reference strainsor isolates | Type | Swarming (mm) | Swimming (mm) | Twitching (mm) | |||
|---|---|---|---|---|---|---|---|
| Control | bio-AgNP | Control | bio-AgNP | Control | bio-AgNP | ||
| PAO1 | C | 58.9 ± 10.4 | 45.7 ± 19.1** | 41.0 ± 2.5 | 29.3 ± 3.6*** | 11.6 ± 0.5 | 11.0 ± 0.8 |
| PA14 | C | 13.9 ± 4.8 | 45.0 ± 20.8** | 34.7 ± 4.0 | 36.1 ± 3.6 | 13.7 ± 1.7 | 19.1 ± 1.1** |
| P1 | E | 38.8 ± 16.1 | 47.3 ± 22.2 | 43.9 ± 2.5 | 46.3 ± 5.2 | 4.2 ± 2.1 | 4.4 ± 1.9 |
| P2 | E | 19.0 ± 8.2 | 21.4 ± 5.4 | 38.7 ± 6.0 | 48.8 ± 10.6** | 5.6 ± 1.0 | 5.2 ± 1.3 |
| P3 | E | 10.1 ± 0.6 | 15.6 ± 4.8** | 41.2 ± 3.4 | 46.3 ± 10.6 | 5.0 ± 1.6 | 5.2 ± 1.3 |
| P4 | E | 17.0 ± 5.2 | 25.6 ± 14.9 | 34.1 ± 7.3 | 34.8 ± 9.8 | 5.0 ± 1.1 | 6.3 ± 1.1** |
| P5 | E | 39.1 ± 20.4 | 44.2 ± 25.0 | 41.1 ± 3.6 | 49.7 ± 13.9 | 4.9 ± 0.9 | 5.3 ± 1.0** |
| P6 | non-CF | 10.9 ± 0.8 | 32.1 ± 29.2** | 41.6 ± 7.6 | 43.3 ± 11.1 | 9.1 ± 1.9 | 12.1 ± 5.6* |
| P7 | non-CF | 8.7 ± 0.7 | 13.2 ± 1.2** | 49.6 ± 5.0 | 46.2 ± 8.9 | 3.5 ± 0.9 | 5.0 ± 1.3* |
| P8 | non-CF | 13.3 ± 2.4 | 17.8 ± 4.2** | 33.8 ± 3.6 | 44.3 ± 4.0*** | 5.1 ± 0.3 | 6.3 ± 3.3* |
| P9 | non-CF | 14.0 ± 1.5 | 28.6 ± 8.2** | 41.2 ± 3.2 | 53.4 ± 10.6* | 7.1 ± 3.3 | 8.1 ± 4.9 |
| P10 | non-CF | 16.4 ± 6.4 | 47.8 ± 8.7*** | 25.2 ± 7.3 | 36.2 ± 6.0** | 2.8 ± 1.3 | 3.9 ± 0.9 |
| P11 | CF | 16.4 ± 6.9 | 20.6 ± 6.1 | 42.2 ± 2.5 | 48.6 ± 9.2* | 7.1 ± 0.8 | 6.2 ± 1.7 |
| P12 | CF | 32.7 ± 21.6 | 25.8 ± 13.2 | 42.6 ± 5.4 | 48.9 ± 8.1* | 5.6 ± 1.1 | 5.3 ± 1.6 |
| P13 | CF | 68.3 ± 15.5 | 73.0 ± 13.3 | 38.4 ± 5.1 | 43.0 ± 10.7 | 4.0 ± 0.6 | 3.6 ± 1.9 |
| P14 | CF | 53.6 ± 30.0 | 52.9 ± 30.5 | 40.3 ± 8.7 | 39.3 ± 15.4 | 4.1 ± 0.7 | 4.8 ± 0.6** |
| P15 | CF | 43.9 ± 30.6 | 41.2 ± 27.8 | 45.8 ± 6.5 | 46.9 ± 9.1 | 4.1 ± 0.9 | 4.4 ± 1.2** |
The results were obtained by the mean ± standard deviation of three independent experiments.
*P < 0.05, ** < 0.01, and *** < 0.001, comparing the control bacteria (without treatment) with the treated (bio-AgNP).
Bio-AgNP, silver nanoparticles.
C, clinical isolates.
E, environmental isolates.
Non-CF, clinical isolates obtained from non-cystic fibrosis patients.
CF, clinical isolates obtained from cystic fibrosis patients.