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. 2021 Apr 1;11:652432. doi: 10.3389/fcimb.2021.652432

Figure 4.

Figure 4

vscF and vscI are involved in the secretion of VPA0226 in V. parahaemolyticus. Intracellular cAMP (A) and the translocated amounts of CyaA-fused protein (B) were quantified after infection with V. parahaemolyticus vscF and vscI mutant strains. GAPDH served as internal reference. “+” or “-” refers to a strain harboring pMMB207-vpa0226-CyaA expression vector (+) or not (-). Densitometry analysis was performed for relative quantification (C). ****P < 0.0001, ***P < 0.001, and **P < 0.01. Relative mRNA transcription levels of vpa0226 genes in POR-1, ΔvscF and CΔvscF strains. For each sample, the acquired cycle threshold (CT) was normalized to the CT of the internal housekeeping gene 16s RNA, and the ΔCT was normalized to the ΔCT of the POR-1 strain. Relative fold differences in mRNA expression level were calculated using the 2−ΔΔCT method (D). Schematic illustration of the conserved domains of the predicted effector encoded by vpa0226. The predicted domains of VPA0226 harboring the typical GDLS lipase are indicated (E). ns, indicates no significant difference.