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S151 of VPA0226 was a significant active site. The transmembrane helices of VPA0226 were predicted to contain 1–23 signal peptides in N-Terminus using Phyre2 (A). The cytotoxicity of V. parahaemolyticus vpa0226-related mutant strains were examined using infected HeLa cells (B). ***P < 0.001, **P < 0.01. ns, indicates no significant difference.
