Table 1.
Plasmids and bacterial strains.
| Strains or plasmids | Characteristics | Source or reference |
|---|---|---|
| Plasmid | ||
| pYAK1 | R6K-ori suicide vector containing sacB gene | (Kodama et al., 2002) |
| pYAK1-ΔvscF | Suicide vector pYAK-1 containing up- and downstream DNA franking sequences to the vscF gene for generating the vscF mutant | This study |
| pYAK1-CΔvscF | Suicide vector pYAK-1 containing vscF gene with a single nucleotide base synonymous mutation for generating the vscF complementation strains | This study |
| pYAK1-ΔvcrD1 | Suicide vector pYAK-1 containing up- and downstream DNA franking sequences to the vcrD1 gene for generating the vcrD1 mutant | (Park et al., 2004b) |
| pYAK1-ΔvcrD2 | Suicide vector pYAK-1 containing up- and downstream DNA franking sequences to the vcrD2 gene for generating the vcrD2 mutant | (Park et al., 2004b) |
| pYAK1-Δvpa0226 | Suicide vector pYAK-1 containing up- and downstream DNA franking sequences to the vpa0226 gene for generating the POR-1-Δvpa0226, POR-2-Δvpa0226, and POR-3-Δvpa0226 mutants | This study |
| pYAK1-S151A | Suicide vector pYAK-1 containing the vpa0226 DNA sequence with 151Ser mutating to Ala for generating the POR-1-S151A, POR-2-S151A, and POR-3-S151A mutants | This study |
| pMMB207 | Expression vector for V. parahaemolyticus, Cmr | (Morales et al., 1991) |
| pMMB207-vp1683-CyaA | Derivative of pMMB207, encoding a fusion of VP1683 with the catalytic domain of adenylate cyclase (CyaA) at the C-terminus from Bordet Ella pertussis | This study |
| pMMB207-vp1686-CyaA | Derivative of pMMB207, encoding a fusion of VP1686 with the catalytic domain of adenylate cyclase (CyaA) at the C-terminus from Bordet Ella pertussis | This study |
| pMMB207-vpa0226-CyaA | Derivative of pMMB207. encoding a fusion of VPA0226 with the catalytic domain of adenylate cyclase (CyaA) at the C-terminus from Bordet Ella pertussis | This study |
| Strain | ||
| E. coli | ||
| SM10λpir | thi thr leu tonA lacy supE recA:RP4-2-Tc: Mu λpir, OriT of RP4 Kmr; conjugational donor | (Miller and Mekalanos, 1988) |
| SM10λpir/1683CyaA | SM10λpir containing plasmid pMMB207- vp1683-CyaA | This study |
| SM10λpir/1686CyaA | SM10 λpir containing plasmid pMMB207- vp1686-CyaA | This study |
| SM10λpir/0226CyaA | SM10 λpir containing plasmid pMMB207- vpa0226-CyaA | This study |
| V. parahaemolyticus | ||
| KXV237 | RIMD 2210633(KP positive, serotype O3:K6) | (Makino et al., 2003) |
| POR-1 | ΔtdhAS derivative of KXV237 | (Park et al., 2004a) |
| POR-1/VP1683-CyaA | POR-1 containing plasmid pMMB207- vp1683-CyaA | This study |
| POR-1/VP1686-CyaA | POR-1 containing plasmid pMMB207-vp1686-CyaA | This study |
| POR-1/VPA0226-CyaA | POR-1 containing plasmid pMMB207-vpa0226-CyaA | This study |
| POR-1-Δvpa0226 | POR-1 knockout of vpa0226 | This study |
| POR-1-S151A | POR-1-Δvpa0226 complementation strains with mutant VPA0226 S151A | This study |
| POR-2 | vcrD1 knockout of POR-1(ΔT3SS1 strain) | (Park et al., 2004b) |
| POR-2/VPA0226CyaA | POR-2 containing pMMB207-vpa0226-CyaA | This study |
| POR-2/VP1683-CyaA | POR-1 containing plasmid pMMB207- vp1683-CyaA | This study |
| POR-2/VP1686-CyaA | POR-1 containing plasmid pMMB207-vp1686-CyaA | This study |
| POR-2-Δvpa0226 | POR-2 knockout of vpa0226 | This study |
| POR-2-S151A | POR-2-Δvpa0226 complementation strains with mutant VPA0226 S151A | This study |
| POR-3 | vcrD2 knockout of POR-1 (ΔT3SS2 strain) | (Park et al., 2004b) |
| POR-3/VP1683-CyaA | POR-1 containing plasmid pMMB207- vp1683-CyaA | This study |
| POR-3/VP1686-CyaA | POR-1 containing plasmid pMMB207-vp1686-CyaA | This study |
| POR-3/VPA0226CyaA | POR-3 containing pMMB207-vpa0226-CyaA | This study |
| POR-3-Δvpa0226 | POR-3 knockout of vpa0226 | This study |
| POR-3-S151A | POR-3-Δvpa0226 complementation strains with mutant VPA0226 S151A | This study |
| ΔvcrD1/ΔvcrD2 | vcrD1/vcrD2 knockout of POR-1 (ΔT3SS1/ΔT3SS2 strain) | (Kodama et al., 2007) |
| ΔvcrD1/ΔvcrD2/VPA0226CyaA | ΔvcrD1/ΔvcrD2 containing plasmid pMMB207-vpa0226-CyaA | This study |
| ΔvscF | POR-1 knockout of vscF (deletion from nt 28 to 250 of the gene) | This study |
| ΔvscF/VP1683-CyaA | ΔvscF containing pMMB207- vp1683-CyaA | This study |
| ΔvscF/VP1686-CyaA | ΔvscF containing pMMB207-vp1686-CyaA | This study |
| ΔvscF/VPA0226-CyaA | ΔvscF containing pMMB207-vp1686-CyaA | This study |
| CΔvscF | vscF complementation strains | This study |
| CΔvscF/VP1683-CyaA | CΔvscF containing pMMB207- vp1683-CyaA | This study |
| CΔvscF/VP1686-CyaA | CΔvscF containing pMMB207-vp1686-CyaA | This study |
| C△vscF/VPA0226-CyaA | CΔvscF containing pMMB207-vp1686-CyaA | This study |
| ΔvscI | POR-1 knockout of vscI | This study |
| ΔvscI/VPA0226-CyaA | ΔvscI containing pMMB207-vp1686-CyaA | This study |
| CΔvscI | vscI complementation strains | This study |
| CΔvscI/VPA0226-CyaA | CΔvscI containing pMMB207-vp1686-CyaA | This study |