Table 2.
Proteins with strong modification of EV‐associated secretion (M score > 1) with high reproducibility (R > 0.9) upon HIV infection of Jurkat cells (top right quadrant of Fig 4C). Estimated FDR = 8%.
| Gene name | Major subcellular localization | Known functions | Movement towards (IN) or away from (OUT) HIV cluster (M/R scores) |
|---|---|---|---|
| ABR | Cytosolic | Regulates GTPases RAC1, RAC2, CDC42 | OUT (3.05/0.958) |
| ALYREF | Nuclear/cytosolic | Nuclear export of mRNA | IN (5.67/0.991) |
| ATP6V0A1 | Multipass transmembrane, intracellular acidic compartment (?) | H+ translocation for acidification of vacuole | IN (1.14/0.983) |
| BROX | Cytosolic membrane associated? (CAAX motif) | IN (1.40/1) | |
| BYSL | Cytosol and nucleolus | rRNA processing | Lateral (1.46/0.940) |
| CARM1 | Nucleus (cytoplasm during mitosis) | Histone methylation | IN (1.13/0.958) |
| CHUK | Cytosol and nucleus | Part of the IKK complex that inhibits NFkB | Lateral (1.01/0.98) |
| CLASP2 | Cytoskeleton, plasma membrane, Golgi | Stabilizes dynamic microtubules | Lateral (1.47/1) |
| CPD | Single‐pass transmembrane, Plasma membrane | Releases C‐term Arg and Lys from polypeptides | Lateral (1.11/0.968) |
| EIF3G | Nucleus, cytoplasm | Required for initiation of protein synthesis by associating with 40S ribosome | Lateral (1.61/0.994) |
| FAM129B = NIBAN2 | Cytosol | OUT (1.39/0.987) | |
| HSPA6/HSPA7 | Cytoplasm and extracellular EVs | Chaperone | Lateral (1.87/0.960) |
| MAD2L1 | Nucleus and mitotic spindle during mitosis | Part of the complex that prevents anaphase until all chromosomes are aligned during mitosis | Lateral (2.26/0944) |
| MOV10 | Nucleus and cytoplasmic granules (P‐Body...) | RNA helicase, required for miRNA‐mediated gene silencing | IN (10.55/1) |
| MYBBP1A | Nucleus and cytoplasm | May activate or repress transcription | IN (2.01/0.982) |
| NEU3 | Peripheral membrane (no signal peptide: cytosolic?) | Modulates ganglioside content at the lipid bilayer | OUT (1.87/0.96) |
| RBM14 | Nucleus, cytosol | Transcriptional activator or repressor (2 isoforms), participate in innate immune response via cGAS‐STING | Lateral (1.28/0.949) |
| SERINC3 | Multipass transmembrane, plasma membrane and Golgi | HIV restriction factor, inhibits early step of viral infection | OUT (1.26/ 0.908) |
| SH3KBP1 | Cytosol, endocytic vesicles | Regulation of endocytosis and receptor signalling, interacts with PDCD6IP | IN (1.63/0.954) |
| SPN | Single‐Pass Type I transmembrane, plasma membrane | Regulates T‐cell functions | IN (1.41/1) |
| TBC1D10C | Cytosol, plasma membrane filopodia | Inhibits Ras signalling and calcineurin | Lateral (2.09/0.987) |
| TMEM106B | Single‐pass type II transmembrane, Lysosomes, endosomes | Regulates lysosomal trafficking via interaction with MAP6 | IN (1.68/0.994) |
| TUBA1 | Cytoskeleton | Constituent of microtubules | OUT (2.41/0.988) |
| TYK2 | Cytosol and extracellular vesicles | Phosphorylates IFNa/b‐Receptor | OUT(1.28/0.998) |
| UBE4B | Nucleus, cytoplasm | Ubiquitin‐protein ligase (E3 ligase) | Lateral (1.16/0.978) |
| ZZEF1 | Unknown (no signal peptide) | Lateral (1.15/0.938) |
Bold formatting indicates proteins that were followed up in detail in this study.