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. 2021 Apr 1;11:644117. doi: 10.3389/fonc.2021.644117

Figure 3.

Figure 3

LC-C upregulates ERRFI1 in A549-IR cells. (A) Heatmap of RNA-seq analysis showing altered transcriptome profiling in A549-IR cells treated with LC-C. Red color in the heatmap denotes upregulation and blue color denotes down-regulation. (B) Volcano plot showing the distribution of -log10 (FDR) vs. log2 (Fold Change). Each point represents a gene, red and green points indicate p value < 0.05, while gray points indicate that p value ≥ 0.05. (C) KEGG pathways enrichment analysis. (D) Graph showing expression changes of the top 20 differentially expressed genes from the RNA-seq analysis; (E) RT-qPCR validation of BCL10, ERRFI1, GA45A, and PLK3 upregulation in A549-IR cells after treatment with LC-C. (F) Western blotting showing the overexpression of ERRFI1 in A549-IR cells. Results shown are the means ± standard deviation. *Significant difference at P < 0.05 level. **Significant difference at P < 0.01 level.