Fig. 7.

csCAF were detected in human PDAC tissue by RNA ISH and IF. (a) The representative immunofluorescence (IF) image for co-staining of COL1A1 (red) and C3 (green) in human PDAC sections with different stages (3 PDAC I, 3 PDAC II, 3 PDAC III), the nuclear were stained with DAPI (blue), T, tumor glands, arrows were pointing to examples of csCAFs. (b) The representative RNA in situ hybridization (RNA ISH) image for co-staining of COL1A1 (green) and C3 (red) in human PDAC sections. (c-d) Bar plot showing the relative number of csCAFs in PDAC with different stages in IF in the c and in RNA ISH in the d. (e-f) The relative expression level of C3 (e) and C7 (f) in PDAC tissue with differnet stages by RT-qPCR. (g) Illustrated summary of CAF subpopulations and their location in PDAC. The marker genes of each CAF subpopulation were listed in corresponding boxes, csCAFs played a vital role in tumor microenvironment by secreting complement (C3 and C7).