Table 2.

Marker panel for imaging mass cytometry.

Target	Clone	Metal	Vendor	Isotype	Dilution	Description
CD3 CD4 Foxp3 CD8a CD45RA CD45RO CD25 CD19 CD33 CD56 CD68 CD11b/Mac-1 CD11c CD14 CD16 TNFα IFN-γ TdT CK AE1/AE3 CD127 Ki-67	Polyclonal, C-Termina EPR6855 206D D8A8Y MEM-56 UCH-L1 EPR6452 6OMP31 Polyclonal RNL-1 KP1 EPR1344 Polyclonal EPR3653 EPR16784 Polyclonal Polyclonal 7UNA8U6 AE-1/AE-3 EPR2955(2) Ki-67	170Er 156Gd 155Gd 162Dy 143Nd 166Er 175Lu 142Nd 145Nd 152Sm 159Tb 149Sm 154Sm 144Nd 146Nd 160Gd 147Sm 141Pr 164Dy 168Er 168Er	Fluidigm Fluidigm Biolegend Fluidigm Abcam Abcam Abcam Fluidigm Fluidigm Abcam Biolegend Fluidigm Fluidigm Fluidigm Fluidigm Abcam Abcam ebioscience Biolegend Fluidigm Biolegend	Polyclonal IgG IgG1, κ IgG IgG2b IgG2a IgG IgG2a IgG IgG1 IgG1, κ IgG Polyclonal IgG IgG Polyclonal IgG IgG1, κ IgG1, κ IgG IgG1, κ	50 50 50 50 100 100 50 50 50 50 200 50 50 50 50 50 50 50 400 50 50	T and NKT cells CD4+ T cells Treg cells CD8+ T cells Naïve T cells Memory T cells IL-2Rα B cells Myeloid cells NK and NKT cells Macrophages Macrophages Dendritic cells Monocytes NK and monocytes Cytokine Cytokine Immature B, T-cells Tumor biomarker IL-7Rα Proliferation marker