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. 2021 Apr 15;101(7):851–864. doi: 10.1038/s41374-021-00593-7

Fig. 5. 5-HT induced a sustained increase in [Ca2+]i in HEK cells co-expressing 5-HT2A receptor and TRPV4.

Fig. 5

A Time traces showing responses to 5-HT (100 μM) by HEK cells expressing 5-HT1A, 5-HT1B or 5-HT2A alone (circles) or with coexpression of TRPV4 (squares). B Time traces showing responses to 5-HT (100 μM) by HEK cells expressing 5-HT2A or coexpressing 5-HT2A/TRPV4. 5-HT-induced [Ca2+]i was abolished by ketanserin (10 μM). C Time traces showing responses to 5-HT (100 μM) by HEK cells expressing 5-HT4 alone or with coexpression of TRPV4. D Effect of the depletion of extracellular Ca2+, PLA2 inhibitor YM 26734 (30 μM), or PKC inhibitor GF 109203X (GFX; 100 nM) in HEK cells co-expressing 5-HT2A/TRPV4. E, F Area under the curve analysis from 60 to 100 s post 5-HT (100 μM) addition. Data are expressed as mean ± SEM for n = 5–6 independent replicates. *p < 0.05; **p < 0.01; One-way ANOVA and Dunnett’s multiple comparisons test. * Indicates statistical significance compared to HEK cells expressing 5-HTR subtype, # indicates statistical significance compared to HEK co-expressing 5-HTR and TRPV4.