Table 7.
Comparison of the mean quantitative polymerase chain reaction mean threshold cycles ± SD between genomic DNA extracted from paired frozen and formalin-fixed paraffin embedded samples
|
F. nucleatum nusG |
16S rRNA |
Human SLCO2A1 |
||||
| Frozen | FFPE | Frozen | FFPE | Frozen | FFPE | |
| Tumor A | 25.9 ± 1.2 | 35.4 ± 0.1 | 21.8 ± 0.1 | 31.4 ± 0.1 | 32.6 ± 2.5 | 39.3 ± 0.6 |
| Nontumor A | 37.2 ± 0.6 | 39.9/ND | 22.2 ± 0.1 | 31.7 ± 0.3 | 34.8 ± 0.5 | ND |
| Tumor B | 26.0 ± 1.0 | 27.5 ± 1.0 | 21.6 ± 0.2 | 28.3 ± 0.1 | 33.2 ± 1.0 | 32.5 ± 1.3 |
| Nontumor B | 31.5 ± 1.4 | 35.1 ± 0.7 | 25.2 ± 0.1 | 31.2 ± 0.6 | 34.6 ± 0.9 | 38.2 ± 0.1 |
F. nucleatum: Fusobacterium nucleatum; FFPE: Formalin-fixed paraffin embedded; ND: Not detected.