Table 1. Quantitation, Dilution Linearity, and Recovery of CatB in Q-Buffera.
| Two-Step Protocol |
Three-StepProtocol |
|||||||
|---|---|---|---|---|---|---|---|---|
| C Spike (pg/mL) | C Det. (pg/mL) | SD (pg/mL) | Error (%) | Recovery (%) | C Det. (pg/mL) | SD (pg/mL) | Error (%) | Recovery (%) |
| 1200.00 | 1190 | 14.03 | 0.83 | 99.17 | 1167 | 20.92 | 2.75 | 97.25 |
| 600.00 | 614 | 35.16 | 2.33 | 102.33 | 587 | 11.57 | 2.17 | 97.83 |
| 300.00 | 311 | 9.85 | 3.67 | 103.67 | 289 | 8.65 | 3.67 | 96.33 |
| 150.00 | 149 | 12.57 | 0.67 | 99.33 | 144 | 3.24 | 4.00 | 96.00 |
| 75.00 | 80 | 6.01 | 6.67 | 106.67 | 68 | 1.26 | 9.33 | 90.67 |
| 37.50 | 39 | 3.90 | 4.00 | 104.00 | 35 | 0.59 | 6.67 | 93.33 |
| 18.75 | 18 | 1.02 | 4.00 | 96.00 | 17 | 0.36 | 9.33 | 90.67 |
| 9.38 | 10 | 0.72 | 6.67 | 106.67 | 8.9 | 0.36 | 5.07 | 94.93 |
| 4.69 | 5.2 | 0.68 | 10.93 | 110.93 | 0 | 0.00 | 100.00 | 0.00 |
| 2.34 | 2.64 | 0.29 | 12.64 | 112.64 | 0 | 0.00 | 100.00 | 0.00 |
| 1.17 | 1.61 | 0.35 | 37.39 | 137.39 | 0 | 0.00 | 100.00 | 0.00 |
| 0.00 | 0.00 | 0.00 | N/A | N/A | 0 | 0.00 | N/A | N/A |
| Extrapolated LOD | 1.56 | Extrapolated LoD | 8.47 | |||||
a
CatB (0–1200 pg/mL) was spiked into the Q-buffer. Samples were serially diluted (1/2) in the Q-buffer. The expected (C Spike) and observed (C Det.) concentrations (pg/mL) with standard deviation (SD), percent (%) of error, and recovery are displayed, as well as the extrapolated limit of detection (LoD, pg/mL) as defined by the SiMoA for two-step and three-step assays are shown.