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. 2021 Mar 31;6(14):9537–9548. doi: 10.1021/acsomega.1c00010

Figure 1.

Figure 1

Analysis of biotransformation products converted by Fusarium sp. CPCC 400226. Products converted from TS were analyzed by thin layer chromatography (TLC). The developing solvents were (A) chloroform/methanol/water (70:26:6, v/v) and (B) petroleum ether/ethyl acetate (2:1, v/v). S1–S5, standard contrast of trillin, prosapogenin A of dioscin, deltonin, zingiberensis newsaponin, and diosgenin, respectively. P1 is the product of TS converted by CPCC 400226 in the YPG medium. Products converted from zingiberensis newsaponin were analyzed by high-performance liquid chromatography-evaporative light-scattering detector (HPLC-ELSD). The blue arrow and the red arrow indicate the substrate of zingiberensis newsaponin (C) and the resulting product of diosgenin (D), respectively.