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| actual
experimentation value |
|||
|---|---|---|---|
| variables | symbol | low (−1) | high (+1) |
| glass bead addition (%) | A (beads) | 2.4 | 3.9 |
| antifoam reagent addition (%) | B (foam) | 0.05 | 0.4 |
| surfactant addition (g/L) | C (surfactant) | 1.5 | 2.5 |
| working volume (mL) | D (volume) | 10 | 30 |
| agitation (rpm) | E (agitation) | 150 | 240 |
| culture temperature (°C) | F (temp) | 22.5 | 33.5 |
| fermentation period (days) | G (period) | 3 | 14 |
| fermentation pH | H (pH) | 4.5 | 6.5 |
| inoculum size (%) | J (inoculum) | 2 | 10 |
