Fig. 5. In vitro autoradiography on sarkosyl-insoluble fraction to assess [125I]61 binding. (a) Autoradiograms showing the binding of [125I]61 (1 nM) in sarkosyl-insoluble fraction from A53T mouse brain. Non-specific binding was defined using unlabeled 61 (100 nM). (b) Quantification of autoradiograms (n = 4); t-test was performed, where *P < 0.0332, **P < 0.0021, ***P < 0.0002 and ****P < 0.0001.
