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. 2021 Apr 7;23(6):428. doi: 10.3892/mmr.2021.12067

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — CCK8 and reverse transcription-quantitative PCR. (A) CCK8 assay showed that 40 µg/ml ozone decreased cell viability by 71%. Changes in mRNA levels of (B) NRF2, (C) p62, (D) HO-1 and (E) LC3 in each group. β-actin was used as the internal control. The data are presented as the mean ± SD (n=3). *P<0.05 vs. 0 µg/ml ozone; ^&P<0.05 vs. 30 µg/ml ozone; ^#P<0.05 vs. 40 µg/ml ozone. T40, 40 µmol/l tBHQ + 40 µg/ml ozone; T0, 40 µmol/l tBHQ; CCK8, Cell Counting Kit-8; NRF2, nuclear factor (erythroid-derived-2)-related 2 factor; HO-1, heme oxygenase 1; tBHQ, tert-butylhydroquinone.