Table B. ICAP nomenclature for the description of fluorescence patterns and corresponding clinical significance.

ICAP code	Fluorescence pattern	Clinical significance*
	Nuclear
AC-1	Nuclear homogenous fluorescence	SLE, drug-induced lupus, JIA
AC-2	Nuclear dense fine speckled	Rare in SjS, SSc, and SLE, more often in healthy individuals and different non-autoimmune inflammatory diseases (atopic dermatitis, asthma, etc.)
ICAP code	Fluorescence pattern	Clinical significance*
AC-3	Centromere fluorescence	Limited cutaneous SSc, PBC
AC-4	Nuclear fine speckled fluorescence	SjS, SLE, dermatomyositis
AC-5	Nuclear large/coarse speckled	MCTD, SLE, SSc
AC-6	Fluorescence of multiple nuclear dots	PBC, SARDs, dermatomyositis
AC-7	Fluorescence of few nuclear dots	SjS, SLE, SSc, polymyositis, asymptomatic individuals
AC-8	Homogenous nucleolar fluorescence	SSc, SSc/PM overlap syndrome
AC-9	Clumpy nucleolar fluorescence	SSc
AC-10	Punctate nucleolar fluorescence	SSc, SjS
AC-11	Smooth nuclear envelope fluorescence	SLE, SjS, seronegative arthritis
AC-12	Punctate nuclear envelope fluorescence	PBC
AC-13	PCNA-like fluorescence	SLE, other disorders
AC-14	CENP-F – like fluorescence	Carcinomas, other disorders
AC-29	Topo-I like fluorescence	SSc
	Cytoplasmic
AC-15	Cytoplasmic fibrillar linear fluorescence	MCTD, chronic active hepatitis, cirrhosis, myasthenia gravis, Morbus Crohn, PBC, long-term haemodialysis, rare in SARDs
AC-16	Cytoplasmic fibrillar filamentous fluorescence	Infective or inflammatory disorders, long-term haemodialysis, alcoholic liver disease, SARDs, psoriasis, healthy individuals
AC-17	Cytoplasmic fibrillar segmental fluorescence	Myasthenia gravis, Morbus Crohn, ulcerative colitis
AC-18	Fluorescence of cytoplasmic discrete dots/GW body-like	PBC, SARDs, different neurologic and autoimmune disorders
AC-19	Cytoplasmic dense fine speckled fluorescence	Anti-synthetase syndrome, polymyositis/dermatomyositis, SLE, juvenile SLE, neuropsychiatry SLE
AC-20	Cytoplasmic speckled fluorescence	Anti-synthetase syndrome, polymyositis/dermatomyositis, limited SSc, idiopathic pleural effusion
AC-21	Cytoplasmic reticular fluorescence/AMA	Often in PBC and SSc, rare in other SARDs
AC-22	Cytoplasmic polar/Golgi like fluorescence	Rare in SjS, SLE, RA, MCTD, GPA, idiopathic cerebral ataxia, paraneoplastic cerebral degeneration, virus infections
AC-23	“Rods & Rings “ fluorescence	HCV patients after IFN-γ/ribavirin therapy, rare in SLE, Hashimoto thyroiditis and healthy individuals
	Mitotic
AC-24	Centrosome fluorescence	Rare in SSc, Raynaud syndrome, infection with viruses and mycoplasmas
AC-25	Fluorescence of spindle fiber	Rare in SjS, SLE, and other CTDs
AC-26	NuMA-like fluorescence	SjS, SLE, other disorders
AC-27	Fluorescence of intercellular bridge	Rare in SSc, Raynaud syndrome and malignancies
AC-28	Mitotic chromosomal fluorescence	Rare in discoid lupus erythematosus, chronic lymphatic leukemia, SjS, and polymyalgia rheumatica
*Report should contain description of fluorescence pattern with corresponding AC number, without clinical significance. Instead, link to the ICAP web site where corresponding clinical significance can be found should be given in the comment of the report. ICAP – International consensus on antinuclear antibody pattern. SLE – Systemic lupus erythematosus. JIA – juvenile idiopathic arthritis. SjS – Sjögren syndrome. SSc – Systemic sclerosis. PBC – Primary biliary cholangitis. MCTD – mixed connective tissue disease. SARD – systemic autoimmune rheumatic diseases. PCNA – Antibodies to proliferating cell nuclear antigen. CENP-F – centromere protein F. Scl-70 – 70kDa antigen associated with scleroderma. PM – polymyositis. GW body – G (glycine) and W (tryptophan) containing body. AMA – antimitochondrial antibodies. HCV – hepatitis C virus. GPA – granulomatosis with polyangiitis. IFN-γ – interferon gamma. CTD – connective tissue diseases. NuMA – nuclear mitotic apparatus protein.