Fig. 1.

Schematic overview of the experimental procedure. Experimental stages of the qDGMS method: Step1 – metabolic labelling of cells, harvesting and total protein quantification; Step 2 – optional subcellular fractionation to enrich the samples for the organelle of choice (e.g. mitochondria), separation of sample components by density (i.e. density gradient ultracentrifugation); Step 3 – verification of gradient integrity and resolution (e.g. SDS-PAGE followed by immunoblotting), mass spectrometric (MS²) analysis; Step 4 – data processing using the ComPrAn R package.