Figure 6.

_SEVs secreted by cells with extra centrosomes can promote PDAC invasion

(A) Experimental flowchart.

(B) Representative bright-field images of heterotypic spheroids. Black arrows: invasive protrusions. Scale bar, 100 μm.

(C) Quantification of the percentage of invasion in 3D spheroids. 5 ng/mL TGF-β was used as positive control. Spheroids n_(+PSCs) = 40, n_{(+PSCs TGF-β)} = 34, n_{(+PSCs −DOX SEVs)} = 31, and n_{(+PSCs +DOX SEVs)} = 31.

(D) Confocal images of spheroids composed of cancer cells (expressing H₂B-RFP; magenta) and PSCs (expressing H₂B-GFP; green). Scale bar, 100 μm. Inset depicts higher magnification of invasive protrusion. Scale bar, 20 μm.

For all graphics, error bars represent mean ± SD from three independent experiments. ^∗∗∗∗p < 0.0001, n.s. (p > 0.05). Graph was analyzed using one-way ANOVA with a Kruskal-Wallis post hoc test.