REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit Alexa-conjugated A488	Life Technologies	Cat#A11008; RRID: AB_143165
Mouse Alexa-conjugated A568	Life Technologies	Cat#A11001, RRID: AB_2534069
Rabbit Alexa-conjugated A568	Life Technologies	Cat#A11011, RRID: AB_143157
Mouse α-tubulin	Sigma-Aldrich	Cat#T9026; RRID: AB_477593
Rabbit centrin2 N-17-R	Santa Cruz	Cat#sc-27793-R; RRID: AB_2082359
Mouse LBPA (6C4)	Merck Millipore	Cat#MABT837
Rabbit LC3B (D11) XP	Cell signaling	Cat#3868S; RRID: AB_2137707
Mouse α-SMA	Sigma-Aldrich	Cat#A2547; RRID: AB_476701
Mouse Ki67 Alexa-conjugated A488	BD Biosciences	Cat#561165; RRID: AB_10611866
Rabbit TSG101 [(EPR7130(b)]	Abcam	Cat#ab125011; RRID: AB_10974262
Rabbit CD63	Abcam	Cat#ab68418; RRID: AB_10563972
Mouse CD81 (clone B-11)	Santa Cruz Biotechnology	Cat#sc-166029; RRID: AB_2275892
Mouse ALIX (clone 3A9)	Cell signaling	Cat#2171, RRID: AB_2299455
Mouse Flotillin-1 (clone 18)	Biosciences	Cat#610821; RRID: AB_398140
HRP- anti rabbit secondary	GE Healthcare	Cat#NA934; RRID: AB_772206
HRP- anti mouse secondary	GE Healthcare	Cat#NA931; RRID: AB_772210
Chemicals, peptides, and recombinant proteins
Doxycycline hyclate	Sigma-Aldrich	#D9891
H2O2	Sigma-Aldrich	#H1009
N-acetyl cysteine	Sigma-Aldrich	#A9165
Bafilomycin A1	Sigma-Aldrich	#B1793-10UG
DMEM/F12	Sigma-Aldrich	#D8437
DMEM	Thermo Fisher Scientific	#41966-029
RPMI-1640	Thermo Fisher Scientific	#11875093
Keratinocyte serum free medium (1X)	Thermo Fisher Scientific	#17005042
Opti-MEM® reduced serum medium	Thermo Fisher Scientific	#31985070
DMEM for SILAC	Thermo Fisher Scientific	#88364
Penicillin/Streptomycin	Thermo Fisher Scientific	#15140-122
FBS	Thermo Fisher Scientific	#10500-064
Tet-free FBS	Hyclone	#SH30070.03T
GIBCO FBS, Dialyzed	Thermo Fisher Scientific	#26400044
Blasticidin	Generon	#2805-10
Geneticin (G418)	Thermo Fisher Scientific	#10131027
Puromycin	InvivoGen	#ant-pr-1
Polybrene	Sigma-Aldrich	#H9268
Formaldehyde 16%	Thermo Fisher Scientific	#28908
Alexa Fluor 568 Phalloidin	Life Technologies	#A12380
Hoechst 33342	ThermoFisher Scientific	# H3570
BODIPY® FL N-(2-Aminoethyl) Maleimide	Thermo Fisher Scientific	#B10250
ProLong anti-fade mounting medium	Molecular Probes	#P36934
BSA	Sigma-Aldrich	#A9647
Lipofectamine 2000	Invitrogen	#11668027
Lipofectamine RNAi Max	Invitrogen	#13778075
RIPA Buffer	Thermo Scientific	#89901
Complete Mini Protease Inhibitor Cocktail	Roche	#11836153001
Phosphatase inhibitor Cocktail	Cell Signaling	#5870
Bradford Protein Assay	Bio-Rad	#5000006
Recombinant Human TGF-β1	PEPROTECH	#100-21
Matrigel ® Matrix Basement Membrane	Life Sciences	#354234
Corning Rat Tail High Concentration	Life Sciences	#354249
DTT	VWR Chemicals	#M109; CAS: 3483-12-3
Iodoacetamide	VWR Chemicals	Cat#786-228; CAS: 144-48-9
Trypsin	Sigma-Aldrich	T6567-1MG
L-Arginine	Sigma-Aldrich	A6969-25G
L-Lysine	Sigma-Aldrich	L8662-25G
L-Arginine [U-13C6]	Cambridge Isotopes	CLM-2265-H-0.5
L-Lysine [4,4,5,5-D4]	Cambridge Isotopes	DLM-2640-0.5
L-Arginine [U-13C6, U-15N4]	Cambridge Isotopes	CNLM-539-H-0.5
L-Lysine [U-13C6, U-15N2]	Cambridge Isotopes	CNLM-291-H-0.5
L-Proline	Sigma-Aldrich	P0380-100G
Critical commercial assays
GSH/GSSH-Glo assay	Promega	#V6611
BCA Protein assay	Thermo Fisher Scientific	#23225
Magic Red Cathepsin B kit	Bio-Rad	#ICT937
CellVue Maroon Cell Labeling Kit	Invitrogen	#88-0870-16
Deposited data
SILAC analysis of exosome secretion in response to centrosome amplification	This paper	PRIDE: PXD020984 accessible via PRIDE partner repository (https://www.ebi.ac.uk/pride/archive/)
Experimental models: cell lines
PaTu-8988T	Prof. Hemant Kocher (QMUL)	N/A
PaTu-8988S	Prof. Yaohe Wang (QMUL)	N/A
Panc-1	Prof. Hemant Kocher (QMUL)	N/A
CFPAC-1	Prof. David Pellman (Harvard)	N/A
Capan-1	Prof. Hemant Kocher (QMUL)	N/A
HPAF-II	Prof. Hemant Kocher (QMUL)	N/A
MIA-PaCa-2	Prof. Hemant Kocher (QMUL)	N/A
DEC-hTERT	Prof. Hemant Kocher (QMUL)	N/A
HPDE	Prof. Yaohe Wang (QMUL)	N/A
PS1	Prof. Hemant Kocher (QMUL)	N/A
HEK293M	Prof. David Pellman (Harvard)	N/A
PaTu-8988S.PLK4	This work	N/A
HPAF-II.PLK4	This work	N/A
HPAF-II.PLK4 H2B RFP	This work	N/A
PS1 H2B GFP	Angus Cameron (QMUL)	N/A
Oligonucleotides
siSAS6 on-TARGET smart pool	Dharmacon	#M-004158-02
siNegative Control	QIAGEN	#1027310
Recombinant DNA
pLenti-CMV-TetR-Blast	Addgene	#17492
pLenti-CMV/TO-Neo-DEST.PLK4	25	N/A
pMD2.G VSV-G	Addgene	#12259
psPAX2 Gag-Pol	Addgene	#12260
Other
qEV original Size Exclusion Chromatography (SEC) columns	izon	#SP1
Tube, Thinwall, Ultra-Clear, 38.5 mL, 25 × 89 mm	Beckman coulter	#344058
Amicon® Ultra-15 Centrifugal Filter Unit	Merk	#UFC9010