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. 2021 Apr 7;23(6):424. doi: 10.3892/mmr.2021.12063

Figure 2.

Figure 2.

Expression levels of miR-574-5p, YAF2, Bax and Bcl-2 in chondrocytes. Chondrocytes were divided into Con group, IL-1β group and IL-1β + CTS group after different treatments. (A) Alcian Blue staining was used to detect the differentiation of chondrocytes. Magnification, ×1,000 (B) Differential expression levels of miR-574-5p and YAF2 in chondrocytes were detected via reverse transcription-quantitative PCR. (C) Western blot analysis was used to detect the protein expression levels of YAF2, Bax and Bcl-2 in chondrocytes. (D) Apoptotic rate of chondrocytes was detected via flow cytometry, and (E) the results were quantified. **P<0.01, ***P<0.001 vs. control group; #P<0.05, ##P<0.01, ###P<0.001 vs. IL-1β group. Con, control; CTS, Cryptotanshinone; miR, microRNA; YAF2, YY1-associated factor 2.