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. 2021 Apr 7;23(6):424. doi: 10.3892/mmr.2021.12063

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Copyright: © Yue et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — CTS regulates the methylation of the miR-574-5p promoter. (A) The upstream 2 kb promoter region of miR-574-5p was detected via biogenic analysis. (B) Methylation levels of miR-574-5p promoter in the Blank group, IL-1β group, IL-1β + CTS group and IL-1β + CTS + 5-aza-CdR group were measured using MSPCR. MSPCR, Methylation specific PCR; MSP, methylated; USP, unmethylated; CTS, Cryptotanshinone; miR, microRNA; 5-aza-CdR, 5-aza-2′-deoxycytidine.