Table 2.
Primer sequences and sizes of PCR products used in PCR reactions.1
| PCR reaction | Primer | Target | Primer sequence | PCR product (bp) |
|---|---|---|---|---|
| Quadruplex | chuA.a1 | chuA | 5′-ATGGTACCGGACGAACCAAC-3′ | 288 |
| chuA.2 | 5′-TGCCGCCAGTACCAAAGACA-3′ | |||
| yjaA.1 b | yjaA | 5′-CAAACGTGAAGTGTCAGGAG-3′ | 211 | |
| yjaA.2 b | 5′-AATGCGTTCCTCAACCTGTG-3′ | |||
| TspE4.C2.1 b | TspE4.C2 | 5′-CACTATTCGTAAGGTCATCC-3′ | 152 | |
| TspE4.C2.2 b | 5′-AGTTTATCGCTGCGGGTCGC-3′ | |||
| AceK.f | 5′-AACGCTATTCGCCAGCTTGC-3′ | 400 | ||
| ArpA1.r | arpA | 5′-TCTCCCCATACCGTACGCTA-3′ | ||
| Group E | ArpAgpE.f ArpAgpE.r |
arpA | 5′-GATTCCATCTTGTCAAAATATGCC-3′ 5′-GAAAAGAAAAAGAATTCCCAAGAG-3′ |
301 |
| Group C | trpAgpC.f trpAgpC.r |
trpA | 5′-AGTTTTATGCCCAGTGCGAG-3′ 5′-TCTGCGCCGGTCACGCCC-3′ |
219 |
1
Reference (Clermont et al., 2013).