Table 2.
Characteristics of the three ELISA tests used in the study
| Name used in the current study | Test 1 | Test 2 | Test 3 |
|---|---|---|---|
| Commercial name | IDEXX Q fever Ab test | LSIVetTM Ruminant Q fever Seruma | ID.Vet ID Screen® Q fever indirect multi-species |
| Manufacturer | IDEXX | LSI Life Technologiesa | IDvetb |
| Kit batches used in the current study | D401, E121 |
ELISACOXLS 020, ELISACOXLS 021, ELISACOXLS 023, ELISACOXLS 024 |
565 747 |
| Strain used for antigen production | Isolated from Dermacentor andersoni ticks (Nine Mile reference strain) | Isolated from an ewe | Isolated from a cow |
| Conjugate | Secondary antibodies biding to ruminant IgG | Protein G (biding to IgG of diverse mammalian species) | Protein G (biding to IgG of diverse mammalian species) |
| Interpretation rules according to the manufacturer (ODR: optical density ratio) |
ODR < 30% Negative 30% < ODR < 40% Doubtful 40% < ODR Positive |
ODR < 40% Negative 40% < ODR < 100% Positive + 100% < ODR < 200% Positive + + 200% < ODR Positive + + + |
ODR < 40% Negative 40% < ODR < 50% Doubtful 50% < ODR < 80% Positive 80% < ODR Strong positive |
aTest 2 is currently commercialized by Themofisher Scientific under the commercial name PrioCHECK™ Ruminant Q Fever Ab Plate Kit. bIDVet is now named Innovative Diagnostics.