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. Author manuscript; available in PMC: 2021 Apr 15.
Published in final edited form as: Cell Rep. 2021 Mar 16;34(11):108869. doi: 10.1016/j.celrep.2021.108869

Figure 7. Reinforcement of heme and αKG metabolism, but not the one-carbon pathway, partially restores complex III function in the absence of SFXN1.

Figure 7.

(A) Cell proliferation in full media (with serine), without serine (-ser), and upon supplementation of serine-free media with 15 μM hemin (−ser + hemin) or 1 mM formate (−ser + formate) (mean ± SEM, n ≥ 4).

(B) Complex III activity in DDM-solubilized mitochondria. At 48 h before mitochondrial isolation, cells were switched to media containing galactose only, galactose with 15 μM hemin, or galactose with 1 mM formate (mean ± SEM, n = 6).

(C) Immunoblotting for select respiratory complex subunits using mitochondrial isolates detailed in (B).

(D) Steady-state protein abundance of select respiratory complex subunits in cell lysates. Cells were grown in glucose-based media and treated with the indicated concentration of SA, an inhibitor of heme biosynthesis, for 2 days.

(E and F) Complex III (E) or IV (F) activity in DDM-solubilized mitochondria. At 48 h before mitochondrial isolation, cells were switched to media containing galactose only or galactose with 125 μM SA (mean ± SEM, n = 3).

(G) Complex III activity in DDM-solubilized mitochondria. At 48 h before mitochondrial isolation, cells were switched to media containing galactose only or galactose with 10 mM dimethyl αKG (DMK) (mean ± SEM, n = 6).

(H) Immunoblotting for select respiratory complex subunits using mitochondrial isolates detailed in (G). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; unpaired Student’s t test.