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. Author manuscript; available in PMC: 2021 Apr 15.
Published in final edited form as: Cell Rep. 2021 Mar 16;34(11):108869. doi: 10.1016/j.celrep.2021.108869
Primer name Primer sequence (5′−3′)
SFXN1-F TCTGGAGAACTACCACCAAAC
SFXN1-R CGGGGTACCTTACAATCCCTTATTGAAGT
CNAP-SFXN1-rxn1-F CTAGCTAGCGTTTAAACTTAAGCTTATGGAAGACCAAG
CNAP-SFXN1-rxn1-R GTTTGGTGGTAGTTCTCCAGAAGGCATGTGATGATGGTG
CNAP-SFXN1-rxn2-F TCTGGAGAACTACCACCAAAC
CNAP-SFXN1-rxn2-R CGGGGTACCTTACAATCCCTTATTGAAGT
SFXN1-HA-rxn1-F TACCCTTACGATGTACCGGATTACGCATGAGGTACCCCG
SFXN1-HA-rxn1-R CGGGGTACCTCATGCGTAATCCGGTACATCGTAAGGGTA
SFXN1-HA-rxn2-F CTAGCTAGCATGTCTGGAGAACTACCACC
SFXN1-HA-rxn2-R TACATCGTAAGGGTACAATCCCTTATTGAA
recomb_Nde1-AGK-F GGAATTCCATATGACGGTGTTCTTTAAAAC
recomb_AGK-HindIII-R CCCAAGCTTTCACTGGGTGGGGCTTGTGAG
recomb_Nde1-hANT2-F CTAGCTAGCATGACAGATGCCGCTGTGTCCTTCGCCAAG
recomb_hANT2-HindIII-R ACGCGTCGACCTTATGTGTACTTCTTGATTTCATC
subclone_AGK-F CTAGCTAGCGCCATGACGGTGTTCTTTAAAACGC
subclone_AGK-R GGGGTACCTCACTGGGTGGGGCTTGTGAGCATC
mutate_AGKG126E-rxn1-F TAATACGACTCACTATAGGGAG
mutate_AGKG126E rxn1-R CAGTGTCTCATCTCCTCCTGCAACAATGATCACATC
mutate_AGKG126E-rxn2-F GAGGAGATGAGACACTGCAGGAGGTTGTTACTG
mutate_AGKG126E-rxn1-R TAGGTCCCGTGTATGAGATAG
genotype_HindIII-sfxn1-F CCCAAGCTTGGGACGTAGTGACATTGTAAG
genotype_sfxn1-SacII-R TCCCCGCGGGGAGATAGCAGCCTAACATGA
genotype_HindIII-agk-F CCCAAGCTTGGGAGGCCAGAGGAGAAGTGGTA
genotype_agk-SacII-R TCCCCGCGGGGATGCAGATGCAAAGGTGGGAT
qpcr_mtcyb-F TGAAACTTCGGCTCACTCCT
qpcr_mtcyb-R TGGGCGATTGATGAAAAGGC
qpcr_nd1-F TTCAGCAATTTAGGAACCACCC
qpcr_nd1-R GGTCACACTTAATTTGCCACCAA
qpcr_uqcrc2-F TTCAGCAATTTAGGAACCACCC
qpcr_uqcrc2-R GGTCACACTTAATTTGCCACCAA
qpcr_uqcrfs1-F CTGAATACCGCCGCCTTGAA
qpcr_uqcrfs1-R ATGCGACACCCACAGTAGTTA
qpcr_alas1-F GCCAGGCTGTGAGATTTACT
qpcr_alas1-R CTGAGGTGGCTGACATCATT
qpcr_alad-F GGGTGGAGGGAGAATACTAAAC
qpcr_alad-R GCCTTGGAGTTGGACTACAT
qpcr_cpox-F CAGGGCTGTAGTTCCTTCTTAC
qpcr_cpox-R GGAGTGAAGAGGCCAAACTT
qpcr_fech-F ATGGAGAGAGATGGCCTAGAA
qpcr_fech-R GCTTCCGTCCCACTTGATTA
qpcr_ppox-F GGAGGCTGACCACGTTATTA
qpcr_ppox-R GGCACCAAATGTCCAAATCC
qpcr_slc25a38-F CTATGGACTACGTGGCTTCTTC
qpcr_slc25a38-R TCCTCTCTTGGTCAGGACTT
qpcr_urod-F TTCCAAGCCATCACCCTTAC
qpcr_urod-R GACTAGCCTGAGGTCTCTGATA
mtDNA_ND1-F ACGGGCTACTACAACCCTTC
mtDNA_ND1-R GCCTAGGTTGAGGTTGACCA
mtDNA_B2M-F GGAGAGCTGTGGACTTCGTC
mtDNA_B2M-R ATTCTACAAACGTCGCGTGC