Figure 3. Prdm12^AvilCKO mice have reduced sensation to nociceptive stimuli and chemical pruritogens.

(A) Prdm12^AvilCKO mice show reduced sensitivity to a sharp pin, p < 0.0001.

(B and C) Prdm12^AvilCKO mice show a delayed response to a clip attached to the tail (B) (p = 0.002), and when placed on a cold plate (C) (0–5°C, p = 0.033).

(D) Mutant mice spend less time licking after intraplantar injection of capsaicin, p = 0.0017.

(E) Prdm12^AvilCKO mice showed no differences in response to heat stimuli in the Hargreaves test.

(F) Mutant mice showed similar latencies to response on hot plate at 50°C, 52°C, and 55°C but had a delayed response at 56°C, p = 0.0112. Lick times at 50°C and 56°C were unchanged.

(G) Prdm12^AvilCKO mice show reduced scratch time after intradermal chloroquine injection in the nape of the neck, p < 0.0001.

(H) Scratch time is similarly reduced in Prdm12^AvilCKO mice after intradermal histamine injection in the nape of the neck, p = 0.029.

(I–K) Light touch is normal in Prdm12^AvilCKO mice, which show similar withdrawal thresholds to von Frey hairs (I), responses to dynamic light touch (J), and latency to removal of a piece of tape applied to the plantar surface of the hindpaw (K).

For (A)–(K), n = 18 control and 14 mutant with 50/50 M:F, except for (D) and lick time in (F) (n = 16 control, 16 mutant, 50/50 M:F) and (H) (n = 14 control, 50/50 M:F, 5 mutant, 4F:1M). Data analyzed by two-tailed Student’s t test; results are presented as means ± SEM.