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. 2021 Apr 15;21:409. doi: 10.1186/s12885-021-08134-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — CPT1A levels in breast cancer tissues, cell lines and culture medium. a CPT1A mRNA levels in normal breast tissues (n = 61), invasive ductal breast cancer (IDC) tissues (n = 389) and invasive lobular breast cancer (ILC) tissues (n = 36) according to data from the Cancer Genome Atlas (TCGA) database. b Overall survival rates of breast cancer patients with low (n = 481) or high (n = 481) expression levels of CPT1A were estimated with the Kaplan–Meier method by log-rank test according to data from TCGA database. The Kaplan–Meier curves were drawn by using the GraphPad Prism 5 software. c CPT1A protein levels in immortalized breast epithelial cell line and breast cancer cell lines. β-Actin was used as a control to confirm equal loading of protein. Greyscale values of each protein bands were calculated. The relative fold change of CPT1A band relative to β-Actin band were indicated below CPT1A bands. Corresponding uncropped full-length blots are presented in Supplementary Fig. 1. The full-length blots were cropped between 55 KDa and 72 KDa to separate CPT1A and β-actin protein. d CPT1A protein levels in cell culture medium of indicated cell lines above were detected by an ELISA assay after 72 h incubation. *p < 0.05, **p < 0.001, ***p < 0.0001