Fig. 5.

SMARCA4 couples with PRMT1 to promote CRC cell proliferation through EGFR signaling in HCT116 cells. a Colony formation assay with HCT116 cells transfected with EV (empty vector, MSCV), PRMT1-WT, PRMT1-Δ, SMARCA4, PRMT1-WT + SMARCA4, or PRMT1-Δ + SMARCA4. Representative images (left panels) and quantitative analyses of colony formation (right panels) are shown. b Cell migration assays with HCT116 cells transfected with MSCV, PRMT1-WT, PRMT1-Δ, SMARCA4, PRMT1-WT + SMARCA4, or PRMT1-Δ + SMARCA4. Representative images (left panels) and quantitative analyses of the migrated cells (right panels) are shown. c Colony formation assays from NC or PRMT1-KD transfected HCT116 cells transfected or not with a SMARCA4 expression construct. Representative images (left panels) and quantitative analyses of the colony formation (right panel) are shown. d Cell migration assays from NC or PRMT1-KD transfected HCT116 cells transfected or not with a SMARCA4 expression construct. Representative images (left panels) and quantitative analyses of the colony formation (right panel) are shown. e Colony formation assays and cell migration assays from NC or PRMT1-KD with ectopic expression of TNS4 or EGFR, or both. Representative images (left panels) and quantitative analyses of the colony formation (right panels) are shown. f Western blot analysis of the expression levels of PRMT1 and EGFR signaling pathway downstream molecules p-AKT, AKT, p-ERK, and ERK in HCT116 cells with ectopic expression of TNS4 or EGFR. GAPDH served as a loading control. All results are shown as mean ± s.d. from three independent experiments; **P < 0.01, *P < 0.05 compared with the indicated control