Fig. 3.

miR-145 inhibits PD-L1 expression by directly targeting the 3′-UTR of PD-L1 in HCC cells. a Predicted target sequences of PD-L1 3′-UTR binding to miR-145. b and c Wild type or mutated PD-L1 3′-UTR transfected into HepG2 or Huh-7 cells with miR-145 or negative control (miR-NC). Relative luciferase activity was determined 48 h post-transfection. d and e PD-L1 protein levels in HepG2 and Huh-7 cells transfected with miR-145 mimics, miR-NC, miR-145 inhibitor or inhibitor NC. **P < 0.01; ***P < 0.001