Fig. 5.

miR-145 inhibits EMT, migration, and invasion of HCC cells. a HepG2 and Huh7 cells were transiently transfected with miR-145 mimics or miR-NC. After 48 h post-transfection, western blot analysis was performed to measure levels of E-cadherin and N-cadherin. Expression levels were normalized against β-actin levels. b HepG2 and Huh7 cells were transiently transfected with miR-145 inhibitor or inhibitor NC. After 48 h post-transfection, western blot analysis was performed to measure levels of E-cadherin and N-cadherin. Expression levels were normalized against β-actin levels. c Migration (through the 8-μM pore) or invasion (through the matrigel-coated membrane) of HepG2 or Huh7 cells transiently transfected with miR-145 mimics or miR-NC. Scale bar, 100 μm. d Migration (through the 8-μM pore) or invasion (through the matrigel-coated membrane) of HepG2 or Huh7 cells transiently transfected with miR-145 inhibitor or inhibitor NC. Scale bar, 100 μm