Fig. 4.

AIF3 splicing caused severe neurodegeneration in the forebrain of 3-month-old mice. a Representative overview images of neurodegeneration in mouse brain, including somatosensory cortex, piriform cortex, thalamus and hippocampus (i-ii, i’-ii’, i”-ii”) as well as cerebellum (iii, iii’, iii”). i-iii, drawing images for coronal mouse brain sections. Somatosensory (orange), piriform cortex (pink) and thalamus (blue) are highlighted. i’-iii’, Nissl staining images of the littermate control AIF mouse (AIF^fl/Y/CamKIIα-iCre-). i”-iii”, Nissl staining images of AIF3 splicing mouse (AIF^fl/Y/CamKIIα-iCre+). b Nissl staining of somatosensory cortex (i, i’), piriform cortex (ii, ii’), thalamus (iii, iii’), hippocampus (iv-vii, iv’-vii’) and cerebellum (viii, viii’) from AIF3 splicing mice and their littermate AIF mice. The boxes in i-viii and i’-viii’ are shown at higher magnification. Scale bar, 200 μm. c Quantification of the brain lesion volume (n = 5). Cortex, Somatosensory cortex; Hip, hippocampus; Piriform, piriform cortex. Data are shown as mean ± S.E.M. and analyzed for statistical significance by one-way ANOVA. **** p < 0.0001