Table 2.
Primers used for genotyping and quantitative PCR
| PCR ID | Primers | Sequence | Size (bp) |
|---|---|---|---|
| iCre | CamkIIα-iCre Fw | 5’-GACAGGCAGGCCTTCTCTGAA-3’ | 536 |
| CamkIIα-iCre Re | 5’-CTTCTCCACACCAGCTGTGGA-3’ | ||
| AIFflox Fw1 | 5’-GCTGGACGTAAACTCCTCTTCAGACCT-3’ | Homo: 276 | |
| AIF3geno | AIFflox Fw2 | 5’-CACTGAAAAAGTAAACTTGGCCATAGTGCT-3’ | Het: 276, 447 |
| AIFflox Re | 5’-CCTCTCTGGTCTAAAATGCCAAGCAA-3’ | WT: 447 | |
| mAIF | mAIF-Fw34 | 5’-ACGGTGCGTGGAAGGAAAAG-3’ | 1920 |
| mAIF-Re1953 | 5’-ATCCCGCCAGGGATGGAAAAG-3’ | ||
| mAIF3 | mAIF-Fw2E1/4 | 5’-GAACCGGCTTCCAGCTACAGAG-3’ | 348 |
| mAIF-Re648 | 5’-GTCTGAATTGCAGTGTCTTTG-3’ | ||
| Cytb | Cytochrome b Fw | 5’-TTCTGAGGTGCCACAGTTATTAC-3’ | 180 |
| Cytochrome b Re | 5’-GAAGAGGAGGTGAACGATTGC-3’ | ||
| RPS2 | RPS2 Fw | 5’-CTGACTCCCGACCTCTGGAAA-3’ | 93 |
| RPS2 Re | 5’-GAGCCTGGGTCCTCTGAACA-3’ | ||
| hAIF | hAIF-Fw128 | 5’-TCGTGCGTGAGAGGAAAGG-3’ | 665 |
| hAIF-Re761 | 5’-GTTTGAATCGCAGTGTCTTTG-3’ | ||
| hAIF3 | hAIF-Fw2E1/4 | 5’-GAACCGGCTCCCAGCTTCAGAA-3’ | 338 |
| hAIF-Re761 | 5’-GTTTGAATCGCAGTGTCTTTG-3’ | ||
| R26-F2 | 5’-GCCTCCTGGCTTCTGAGGACCG-3’ | WT: 200 | |
| Rosa26 | R26-R2 | 5’-TCTGTGGGAAGTCTTGTCCCTCC-3’ | KI: 126 |
| SAR-R | 5’-CCTGGACTACTGCGCCCTACAGA-3’ | ||
| AIF3KI | mAIF3KI-F | 5’-GGGATTGTGCTATGGAACGTC-3’ | 812 |
| R26-R2 | 5’-TCTGTGGGAAGTCTTGTCCCTCC-3’ | ||
| Nestin-WT-F | 5’-TTGCTAAAGCGCTACATAGGA-3’ | WT: 246 | |
| Nestin-Cre | Nestin-R | 5’-GCCTTATTGTGGAAGGACTG-3’ | Cre: 150 |
| Nestin-Mu-F | 5’-CCTTCCTGAAGCAGTAGAGCA-3’ | ||
| mAIFGSP1-1951 | 5’-GCTTGTGTATTCCACGATTGGGATTCA-3’ | ||
| RACE | mAIFGSP2-1787 | 5’-GGTAGAAGATGACACCTTTGCCGTAGT-3’ | |
| mAIFGSP3-1009 | 5’-CCCGCCGATAACTGTAATTGACTTGAC-3’ | ||
| mAIFGSP4-608 | 5’-GAGGAGGTCGCATGTATGGCAGTTCAG-3’ |