Fig. 4.
(A) Structures of the most commonly used engineered binding scaffolds derived from the Protein Data Bank (PDB) (DARPin: 4JB8 [107], affibody: 2KZI [108], nanobody: 5F1K [109], Sso7d: 1SSO [110], monobody/adnectin 3QWQ [111], and anticalin: 1L6M [112]). Randomized positions within the respective scaffolds are highlighted in red. (B) Overview of the key features of depicted binding scaffolds. (C) Complementarity‐determining regions (CDRs) in an scFv. The VL domain is colored in light gray, the VH domain in dark gray, and the respective CDRs are colored in dark and light red for the VL and VH domains, respectively. The depicted scFv is directed against IL‐15 (PDB: 2XQB). All graphics were generated using pymol.