Table 1.
Conserved amino acids of fold type IV PLP‐dependent enzymes according to Höhne et al. [8] and corresponding amino acids of the selected new (R)‐ATAs (numbering of amino acid residues according to Höhne et al. [8] ). Shaded amino acids mark underrepresented amino acids of the motif, insertions in the region of the flexible loop and the RxH motif in ES‐ωTA. The arginine (red R) is supposedly the conserved arginine in the flexible loop that is proposed to play a role in dual substrate recognition.
|
Protein |
Sequence motif 1 |
Sequence motif 2 |
|||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
31 |
36 |
38 |
40 |
95 ff |
105 ff |
|
||||||||||||||
|
conserved amino acids |
ADCLs |
[FY] |
F |
T |
X |
zxK |
R |
G |
Y |
|
|
|
|
|
|
|
|
|
|
|
|
|
d‐ATAs |
F |
Y |
V |
K(R/X) |
zYzQ |
R |
x |
H |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
BCATs |
Y |
F |
G |
R(K) |
YzR |
z |
G |
z |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
(R)‐ATAs |
[HR] |
Y |
[VT] |
[STAHP] |
[FY]V[EANQ] |
not conserved (active site loop) |
|||||||||||||||
|
amino acids found in novel putative (R)‐ATAs |
ES‐ωTA |
H |
Y |
V |
S |
FVE |
V |
R |
G |
– |
– |
– |
– |
H |
T |
P |
G |
E |
T |
F |
|
|
FF‐ωTA |
H |
Y |
V |
H |
YVE |
P |
G |
S |
– |
– |
– |
– |
– |
R |
D |
L |
R |
T |
C |
|
|
|
MeV‐ωTA |
R |
Y |
V |
G [a] |
YVA |
A |
G |
A |
P |
– |
– |
– |
– |
Y |
H |
P |
A |
H |
A |
|
|
|
PA‐ωTA |
R |
Y |
V |
A |
YVE |
H |
G |
S |
– |
– |
– |
– |
– |
R |
D |
P |
R |
T |
F |
|
|
|
RA‐ωTA |
R |
Y |
V |
H |
YVE |
Y |
G |
S |
– |
– |
– |
– |
– |
R |
D |
P |
R |
E |
C |
|
|
|
SH‐ωTA |
H |
Y |
V |
A |
YVM[ a] |
V |
R |
Q |
– |
– |
– |
– |
– |
Y |
A |
P |
E |
E |
C |
|
|
|
SM‐ωTA |
R |
Y |
V |
S |
YVC [a] |
V |
S |
N |
N |
P |
A |
I |
G |
K |
K |
A |
E |
E |
L |
|
|
|
TJ‐ωTA |
R |
Y |
V |
A |
YVE |
A |
G |
Q |
– |
– |
– |
– |
– |
R |
D |
P |
R |
L |
L |
||
[a] Indicated bold amino acids are underrepresented amino acids of the (R)‐ATA motifs published by Höhne et al. [8]