TABLE 2.
Gene | PCR primers |
---|---|
GAPDH |
Forward: 5′‐ GCACCGTCAAGGCTGAGAAC ‐3′ Reverse: 5′‐ TGGTGAAGACGCCAGTGGA ‐3′ |
CDX2 |
Forward: 5′‐ TTCACTACAGTCGCTACATCACCA ‐3′ Reverse: 5′‐ CTGCGGTTCTGAAACCAGATT ‐3′ |
Klf4 |
Forward: 5′‐ GTGCCCCGAATAACAGCTCA ‐3′ Reverse: 5′‐ TTCTCACCTGTGTGGGTTCG ‐3′ |
Villin1 |
Forward: 5′‐ CGACTGCTACCTGCTGCTCTACAC ‐3′ Reverse: 5′‐ CGGCTTGATAAGCTGATGCTGTAA ‐3′ |
Cadherin17 |
Forward: 5′‐ AGCAGGTCACCAGACTGGGATAC ‐3′ Reverse: 5′‐ TCAGATGCTTGAGCACTTTCAACA ‐3′ |
MUC2 |
Forward: 5′‐ GATCATAGCACCTTGGATGGG ‐3′ Reverse: 5′‐ GGCACAGTCTGATGACCGG ‐3′ |
FoxO4 |
Forward: 5′‐ TGGGCTCAATCTCACCTCTTCC ‐3′ Reverse: 5′‐ AGAAGCACCCTTCTCCTGCTGA ‐3′ |