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. 2020 Oct 19;99(4):388–398. doi: 10.1002/cyto.a.24229

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© 2020 The Authors. Cytometry Part A published by Wiley Periodicals LLC. on behalf of International Society for Advancement of Cytometry.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Comparison between live and fixed cells (a) global refractive index, (b) surface area of cells in the optimum focus plane (c) relative change of the surface area after fixation. [Color figure can be viewed at wileyonlinelibrary.com]